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Invitrogen™ IEF Marker 3-10

Catalog No. 3921201
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3921201 500 μL
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Catalog No. 3921201 Supplier Invitrogen™ Supplier No. 3921201
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Description

Includes

Supplied in 10% glycerol containing Bromophenol Blue (0.01%) and methyl Red (0.01%)

Ready-to-use protein marker especially developed for IEF applications

The IEF Marker 3-10 Marker works well on all horizontal and vertical one-dimensional IEF gels, and can be used for both native and denaturing conditions (e.g.,8M urea).

  • A ready-to-use format for IEF gels
  • Ideal pI determination of unknown protein samples
  • Monitoring of separation on IEF gels
  • One standard applicable to all IEF gels (vertical/horizontal)
  • 13 purified isoforms — no need for extra “High pI range” or “Low pI range” markers
  • Purified, salt-free protein components
  • Gel Compatibility: Novex IEF Gels

Isoelectric Focusing, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Specifications

Content And Storage The IEF Marker 3-10 is supplied in 10% glycerol containing Bromophenol Blue (0.01%) and Methyl Red (0.01%).

Store in freezer (-5 to -30°C).
Number of Markers 13
Ready to Load Yes
Recommended Applications IEF gels
Size Range 3.5 to 10.7 pI
For Use With (Application) IEF gels
Gel Compatibility Novex™ IEF Gels
Molecular Weight (g/mol) 10.7, 9.5, 8.3, 8.0, 7.8, 7.4, 6.9, 6.0, 5.3, 5.2, 4.5, 4.2, 3.5
Quantity 500 μL
Shipping Condition Dry Ice
Product Type IEF Marker
Stain Type Unstained
System Type IEF
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Frequently Asked Questions (FAQs)

Can I use the IEF Marker, 3-10 for protein quantitation?

The exact amount of each protein in the IEF Marker, 3-10 is not known. We do not recommend using this marker for quantitation purpose.
Can I use the IEF Marker 3-10 with IPG strips?

The IEF Marker, 3-10 is not designed for use with IPG strips.
What are the isoelectric points (pIs) and origins of the proteins in the IEF Marker, 3-10?

The IEF Marker 3-10 is a ready-to-use, unstained protein marker especially developed for IEF applications. It contains 13 purified proteins ranging in pI from 3-10. It works well on horizontal and vertical one-dimensional IEF gels and can be used for both native and denaturing conditions (e.g., 8 M urea, to determine the isoelectric point (pI) of unknown protein samples. Here are the pIs and origins of the proteins in the IEF Marker, 3-10:

- Band no. 9: Cytochrome C; Pig heart origin; 10.7 pI
- Band no. 8 Ribonuclease A; Bovine pancreas origin; 9.5 pI
- Band no. 7 c, m, a Lectin; Lens culinaris origin; 8.3, 8.0, 7.8 pI
- Band no. 6 c, a Myoglobin; Horse muscle origin; 7.4, 6.9 pI
- Band no. 5 Carbonic anhydrase; Bovine erythrocytes origin; 6.0 pI
- Band no. 4 c, a B-Lactoglobulin; Bovine milk origin; 5.3, 5.2 pI
- Band no. 3 Trypsin inhibitor; Soybean origin; 4.5 pI
- Band no. 2 Glucose oxidase; Aspergillus niger origin; 4.2 pI
- Band no. 1 Amyloglucosidase; Aspergillus niger origin; 3.5 pI

Note: Temperature = 5 degrees C for pI values given. pI is temperature dependent, i.e. decreases as temperature increases (approximately -0.005 to-0.03 pH units per degree C)

c = cathodic; m = middle; a = anodic

What are the storage conditions and shelf life for the IEF Marker 3-10?

We recommend storing the IEF Marker 3-10 at -20 degrees C and it is stable for 1 year when properly stored.
My monoclonal antibody runs as a smear on an IEF gel, while the IEF marker focuses perfectly. Why is this?

It is not unusual for antibodies (even monoclonals) to be differentially glycosylated, and therefore not focus well on IEF gels. Sometimes a monoclonal can even run more smeary or unfocused than a polyclonal, over a range of pH 6.5 to 8.0. Try running the gel longer to improve the focus, but often the improvement may be minimal if any at all.

For Research Use Only. Not for use in diagnostic procedures.