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  6. Invitrogen™ Bolt™ Transfer Buffer (20X)

Invitrogen™ Bolt™ Transfer Buffer (20X)

Catalog No. BT00061
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BT0006 125mL
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Description

Requires

A Bolt™ Mini Gel Tank is required for use of all Bolt gels.

Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting

  • When combined with 10% methanol, Bolt Transfer Buffer can be used with Bolt Mini Blot Module for wet transfer
  • Can also be used with Bolt Antioxidant to enhance transfer of reduced proteins to membranes

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

Content And Storage Store at 4 to 25°C.
Buffer Transfer Buffers
Concentration 20 X
Volume (Metric) 1 L
Form Liquid
Gel Compatibility Bolt™ Bis-Tris Plus Gels
Quantity 1L
Product Line Bolt
Product Type Western Blot Transfer Buffer

Frequently Asked Questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.
What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.
How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.
Which power supplies are compatible with the Mini Gel Tank?

The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible
Are Bolt gels compatible with transfer devices from other suppliers?

Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.
Are Bolt gels compatible with other transfer devices?

Yes. You can use the iBlot Dry Transfer System or XCell II Blot Module with SureLock tank and achieve similar transfer efficiency.
The pH of my transfer buffer deviates from the recommended value by 0.2 units. Can I still use the buffer?

We recommend discarding the buffer and remaking it after rechecking the reagents and the water purity. We do not recommend adjusting the pH with acid or base, as this will increase the conductivity of the buffer and result in higher current during the transfer.

For Research Use Only. Not for use in diagnostic procedures.