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  6. Invitrogen™ 4X Bolt™ LDS Sample Buffer

Invitrogen™ 4X Bolt™ LDS Sample Buffer

Catalog No. B0008
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250 mL
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B0008 250 mL
B0007 10 mL
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Catalog No. B0008 Supplier Invitrogen™ Supplier No. B0008
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Description

Includes

lithium dodecyl sulfate (LDS) at pH 8.4.

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

Novex™ Bolt™ buffers and sample reducing agents are optimized for use with Bolt Bis-Tris Plus gels, and are available in a variety of formats.

About Bolt™ Bis-Tris Plus Gels
Bolt™ Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt™ gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt™ gels to obtain optimal results for your day-to-day protein separation needs. Bolt™ Bis-Tris Plus gels come in four gel types and multiple well formats. A Mini Gel Tank is required for use of all Bolt™ gels.

Bolt™ gel run times may vary depending upon the type of running buffer used.

Specifications

Content And Storage Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red.

Store at 4°C to 25°C.
Buffer Sample Loading Buffers
Concentration 4X
Gel Type Bis-Tris Plus
Gel Compatibility Bolt™ Bis-Tris Plus Gels
Quantity 250 mL
Product Line Bolt
Product Type LDS Sample Buffer
For Use With (Equipment) Bolt Mini Gel Tank

Frequently Asked Questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.
What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.
How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.
Which power supplies are compatible with the Mini Gel Tank?

The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible
Are Bolt gels compatible with transfer devices from other suppliers?

Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.
Are Bolt gels compatible with other transfer devices?

Yes. You can use the iBlot Dry Transfer System or XCell II Blot Module with SureLock tank and achieve similar transfer efficiency.
Can I use β-mercaptoethanol as a reducing agent with 4X Bolt™ LDS Sample Buffer (Cat. No. B0007, B0008)? If so, what should the final concentration of β-mercaptoethanol added into the buffer be?

Yes, you can use β-mercaptoethanol at a final concentration of 2.5% (in 1X dilution of the buffer), instead of DTT.

For Research Use Only. Not for use in diagnostic procedures.