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Invitrogen™ Nitric Oxide Assay Kit DFS Item

Measure nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in serum, plasma, urine, saliva and other biological fluids.

Supplier:  Invitrogen™ EMSNO

Catalog No. ENEMSNO



Description

Description

Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.
Additional Information and Online Ordering »

Nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. The interaction of nitric oxide (NO) in a system is measured by the determination of both nitrate and nitrite concentrations in the sample. The relative levels of nitrite and nitrate can vary substantially, therefore the most accurate determination of total NO production requires measurement of both nitrate and nitrite.

  • Target: Nitric oxide (NO) and relative levels of nitrite (NO2-) and nitrate (NO3-)

  • Format: Colorimetric stain (azo dye) for clear 96-well plates

  • Measurement: Absorbance at 540nm (± 20nm)

  • Assay Range: 0 to 100μM

  • Sensitivity: 0.222μM nitrite; 0.625μM nitrate

  • Precision: Dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)

  • Sample Types: Urine, saliva, plasma, serum and other biological fluids

  • Total Assay Time: 15 minutes (nitrite assay); 45 minutes (nitrate assay)

  • Sample Size: 50μL

Includes:

Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.

Specifications

Specifications

0.222µM nitrite, 0.625µM nitrate
Detection Kit
Diazo
• Reagent diluent
• Nitrate reductase diluent
• Nitrate reductase
• NADH
• Nitrite standard
• Nitrate standard
• Griess reagents
• Plates and plate sealer

Store the kit at ≤ –20°C.

See product manual for detailed contents and storage conditions for maximum stability.
Colorimetric
Microplate Reader
SDS
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