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Gibco™ Neurobasal™-A Medium, no D-glucose, no sodium pyruvate
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Catalog No. A2477501
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A2477501 500 mL
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Catalog No. A2477501 Supplier Gibco™ Supplier No. A2477501
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Description

Gibco™ Neurobasal™-A medium without D-glucose and sodium pyruvate is used to support the growth of post-natal CNS neurons in applications where the presence of D-glucose and sodium pyruvate can cause interference.

  • For neural studies that require the absence of insulin, glucose, and sodium pyruvate, such as glucose response or insulin response studies, we recommend Gibco B-27 Supplement Minus Insulin as a supplement to this medium.
  • Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

Specifications

Cell Type Neural Cells
Classification Serum-free
Form Liquid
Product Type Neurobasal Medium
With Additives Phenol Red
Without Additives No Glucose, No Sodium Pyruvate
Product Line Neurobasal
Quantity 500 mL
Shipping Condition Ambient
Tested For Endotoxin, Performance, Sterility, Osmolality, pH
Content And Storage Store in refrigerator (2–8°C) and protect from light.
Culture Type Neuronal Cell Culture
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Frequently Asked Questions (FAQs)

Can you provide references for growing adult neurons?

Instructions for growing adult neurons can be found in the Neurobasal-A Medium product insert. You can also refer to the following publication for growing adult neurons:
• Isolation and Culture of Adult Rat Hippocampal Neurons by Brewer, G.J., J. Neuroscience Methods 71 (1997), 145-158.
Should I use Neurobasal-A Medium with B27 and b-FGF for postnatal neurons?

We do not know at what age postnatal neurons require FGF and a higher osmolality medium, or how this could vary based on the species from which the neural tissue is isolated. Without this knowledge, we generally recommend switching to the Neurobasal-A Medium for isolates from animals above 1 week of age. You will need to determine if the Neurobasal-A Medium is optimal for neurons you have from one day old rats.

Further, the addition of bFGF can increase the viability of neurons of any age. Therefore, caution needs to be made in impure neuronal cell culture as bFGF can increase the proliferation of non-neuronal cells. We recommend a pilot experiment to test the impure culture in the presence and absence of bFGF.
What should I add to Neurobasal-A Medium to grow adult neurons?

We recommend adding B27 and b-FGF to Neurobasal-A Medium to grow adult neurons.
What did you add to Neurobasal-A Medium to increase osmolality?

4 g/L of NaCl has been added to the Neurobasal-A Medium formulation to increase osmolality.

For Research Use Only. Not for use in diagnostic procedures.