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Invitrogen™ eBioscience™ Mouse Cytokine Positive Control Cells

Catalog No. 501122024
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501122024 1 mL
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Catalog No. 501122024 Supplier Invitrogen™ Supplier No. 00450051
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Description

Recommended for use as positive control cells when establishing an intracellular cytokine staining protocol.

  • Mouse splenocytes were stimulated with ConA (3μg/mL) for two days, followed by recombinant IL-2 (20ng/mL) and IL-4 (20ng/mL) for three days
  • The cells were then restimulated with immobilized anti-mouse CD3 (10μg/mL) and soluble anti-mouse CD28 (2μg/mL) in the presence of Brefeldin A for 5 hours
  • These cells contain subpopulations that express IL-2, IL-3, IL-4, IL-10, GM-CSF, and IFNγ
  • The cells have been fixed with formaldehyde and are provided in a stabilizing buffer so that they may be stored at −80°C for up to six months or at 2 to 8°C for up to one month

Specifications

For Use With (Equipment) Flow Cytometer
Quantity 1 mL
Product Line eBioscience
Product Type Mouse Cytokine Positive Control Cells

For Research Use Only. Not for use in diagnostic procedures.