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Invitrogen™ TetraSpeck™ Fluorescent Microspheres Size Kit (mounted on slide)

Catalog No. T14792
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T14792 1 Kit
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Catalog No. T14792 Supplier Invitrogen™ Supplier No. T14792
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Description

For calibration of wide field, TIRF and confocal fluorescence microscopes, especially for multicolor applications like colocalization analysis and derivation of point spread functions critical for deconvolution

  • Contains one microscope slide, with six viewing regions
  • Each of five viewing regions contains microspheres of one size: 0.1, 0.2, 0.5, 1.0 or 4.0μm
  • Remaining region contains mixture of all five bead sizes
  • Every microsphere is stained with four different fluorescent dyes [365/430nm (blue), 505/515nm (green), 560/580nm (orange), and 66/680nm (dark red)] that have well-separated excitation and emission peaks

Cell Analysis, Cell Cycle, Cell Viability, Proliferation and Function, Cellular Imaging, Immunofluorescence (IF)

Specifications

Calibration Type Confocal Microscope Calibration, Fluorescence Microscope Calibration
Content And Storage Store at room temperature and protect from light.
Color Orange, Dark Red, Blue, Green
Product Type Fluorescent Microspheres Size Kit
Format Mounted on Slide
Product Line TetraSpeck
Quantity 1 Kit
Shipping Condition Room Temperature
Diameter (Metric) 0.5, 0.2, 0.1, 1, 4μm

Frequently Asked Questions (FAQs)

What are the excitation/emission peaks for TetraSpeck Microspheres?

The TetraSpeck Microspheres (Cat. Nos. T7279, T7280, T7281, T7283, T7284, T14792) are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks at 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red).

TetraSpeck Blue Dye Spectra
Fluorescence excitation and emission spectra of bead encapsulated TetraSpeck blue dye.
TetraSpeck Blue Dye Spectra

TetraSpeck Orange Dye Spectra


TetraSpeck Green Dye Spectra
TetraSpeck Green Dye Spectra

TetraSpeck Dark Red Dye Spectra
TetraSpeck Dark Red Spectra

For Research Use Only. Not for use in diagnostic procedures.