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Invitrogen™ SYPRO™ Protein Gel Stains
Description
SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE). Stained proteins can be viewed with a standard UV or blue-light transilluminator, or imaging equipment containing the appropriate filters or laser. SYPRO protein gel stains offer many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining, a linear dynamic range over three orders of magnitude, and very little protein-to-protein variation in staining.
SYPRO Ruby Protein Gel Stain features include:
- Convenient—ready-to-use formulation
- High sensitivity—detection to 0.25 to 1 ng
- Effective staining for 1D and 2D gels
- Can be multiplexed with other gel stains such as Pro-Q Diamond phosphoprotein gel stain or Pro-Q Emerald 300 glycoprotein gel stain
- Compatible with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
SYPRO Orange and SYPRO Red protein gel stain features include:
- Sensitive—detection to 4 to 8 ng
- Fast—rapid staining time of 10–60 min
- Best for 1D gels
- SYPRO Orange: Ex: 300, 470 nm, Em: 570 nm
- SYPRO Red: Ex: 300, 550 nm, Em: 630 nm
SYPRO Tangerine Protein Gel Stain features include:
- Sensitive—detection to 4 to 8 ng
- Fast—rapid staining time of 10 to 60 minutes
- Best for 1D gels
- Does not require fixation with acetic acid
- Compatible with subsequent gel staining, western blotting, zymography, electroelution or mass spectrometry
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | Supplied as a 5000X concentrate in DMSO. Store at room temperature and protect from light. |
| Detection Location | In-Gel Detection |
| Color | Orange |
| Concentration | 5000X in DMSO |
| Detection Method | Fluorescence |
| Label or Dye | SYPRO Orange |
| Quantity | 500 μL |
| Shipping Condition | Room Temperature |
| Target Molecule | Protein |
| Excitation/Emission | 300, 470/570 nm |
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