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Invitrogen™ RH 237 (N-(4-Sulfobutyl)-4-(6-(4-(Dibutylamino)phenyl)hexatrienyl)Pyridinium, Inner Salt)

Catalog No. S1109
Encompass
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S1109 5 mg
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Catalog No. S1109 Supplier Invitrogen™ Supplier No. S1109
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Description

Fast-response potential-sensitive probe

RH 237 can be used for imaging functional neurons.

Cell Analysis, Cell Metabolism, Cell Structure, Cell Viability, Proliferation & Function, Membranes (General) & Lipids

Order Info

Shipping Condition: Room Temperature

Specifications

Color Infrared
Detection Method Fluorescence
Product Type Sensitive Probe
Quantity 5 mg
Content And Storage Store at room temperature and protect from light.
Shipping Condition Room Temperature
For Use With (Equipment) Fluorescence Microscope

Frequently Asked Questions (FAQs)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.
What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

For Research Use Only. Not for use in diagnostic procedures.