Invitrogen™ PED6 (N-((6-(2,4-Dinitrophenyl)amino)hexanoyl)-2-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)-1-Hexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) 

Description

Molecular Probes phospholipase substrates are dye-labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells.

• Label (Ex/Em): PED6
• Specificity: PLA₂ (this substrate is cleaved by PLA₁, but this reaction does not separate the fluorophore from the quencher)
• Cleavage product: BODIPY™ FL C5
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry

Note: Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Cellular Imaging, Immunofluorescence (IF), Immunofluorescence Staining and Detection

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