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Invitrogen™ NeuroTrace™ CM-DiI Tissue-Labeling Paste

Catalog No. N22883
Encompass
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N22883 100 mg
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Catalog No. N22883 Supplier Invitrogen™ Supplier No. N22883
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Description

Consists of CM-DiI mixed into an inert, water-resistant gel

  • Ready to use as supplied and can be applied directly to live or fixed tissue specimens using tip of needle
  • Improves penetration of dye into bundled neurons, labeling axons both on and below surface

Cell Analysis, Cell Tracing and Tracking, Neuronal Tracing

Order Info

Shipping Condition: Room temperature

Specifications

Color Yellow
Detection Method Fluorescence
Product Type Tissue Labeling Paste
Quantity 100 mg
Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Shipping Condition Room Temperature
For Use With (Equipment) Fluorescence Microscope
Sub Cellular Localization Cell Membranes & Lipids
Product Line NEUROTRACE

Frequently Asked Questions (FAQs)

I am doing retrograde neuronal labeling using the NeuroTrace DiI Paste. I apply a small glob onto the neuron of interest, but it is not adhering very well. What can I do?

This paste, which has DiI C18 uniformly dispersed within a proprietary hydrophobic carrier, is commonly used to dab onto larger areas than smaller areas commonly used with crystals. Any moisture on the area the paste is applied may be the cause for lack of adherence. Apply the paste using a small flat-head spatula or toothpick and keep it in place a bit longer to ensure contact. The area may also be covered with parafilm to ensure contact as well.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.
How do I know which tracer to choose for my experiment?

Factors to consider are size of tracer, method of delivery (injection, direct application to tissue, etc.), and if the tracer needs to be fixable. Here are some links to details about the various classes of neuronal tracers we offer and how to choose between them:

Neuronal Tracing (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)
Choosing a Tracer (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/choosing-a-tracer.html)
Imaging Analysis (http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/bioprobes-50-journal.pdf)

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

For Research Use Only. Not for use in diagnostic procedures.