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Invitrogen™ HPF (Hydroxyl Radical and Peroxynitrite Sensor) DFS Item

Two indicators that together can selectively detect hypochlorite anion

Supplier:  Invitrogen™ H36004

Catalog No. H36004



Aminophenyl fluorescein (APF, A36003) and hydroxyphenyl fluorescein (HPF), two reactive oxygen species (ROS) indicators developed by T. Nagano, offer greater specificity and stability than dichlorodihydrofluorescein diacetate (H2DCFDA, D399). APF and HPF show limited non-selective reactivity and relatively high resistance to light-induced oxidation.

  • Fluorescein derivatives are nonfluorescent until they react with hydroxyl radical or peroxynitrite anion
  • APF will also react with hypochlorite anion
  • Two ROS indicators can selectively detect hypochlorite anion
  • Upon oxidation, both APF and HPF exhibit bright green fluorescence (excitation/ emission maxima ∼490/515 nm), making them compatible with any instrument that can detect fluorescein

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitro-Oxidative Stress, Reactive Oxygen Species

Order Info

Shipping Condition: Room Temperature





For Research Use Only. Not for use in diagnostic procedures.