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Invitrogen™ FluoCells™ Prepared Slide #3 (mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI)

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Description

Multilabeled cell preparations for observation by fluorescence microscopy validated for immunofluorescence

FluoCells prepared slide #3 is ideal for demonstrating the optical sectioning properties of confocal microscopes. Each slide contains a 16 μm cryostat section of mouse kidney stained with Alexa Fluor 488 wheat germ agglutinin (W-11261), Alexa Fluor 568 phalloidin (A-12380) and DAPI (D-1306).

Cell Analysis, Cell Cycle, Cell Viability, Proliferation and Function, Cellular Imaging, Immunofluorescence (IF)

Order Info

Shipping Condition: Room temperature

Specifications

Type Prepared Microscope Slide
Product Line Alexa Fluor FluoCells
For Use With (Application) Immunofluorescence
Content And Storage Store in freezer -5°C to -30°C and protect from light.
Quantity 1 Each
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?

Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.
DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

For Research Use Only. Not for use in diagnostic procedures.