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Invitrogen™ FluoCells™ Prepared Slide #2 (BPAE cells with Mouse Anti-α-tubulin, AlexaFluor™ 488, FL Goat Anti-Mouse IgG, Texas Red™-X Phalloidin, and DAPI)

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Description

Multilabeled cell preparations for observation by fluorescence microscopy

FluoCells prepared slide #2 contains bovine pulmonary artery endothelial cells (BPAEC). Texas Red-X phalloidin is labeling the F-actin, anti—bovine α-tubulin mouse monoclonal 236-10501 in conjunction with BODIPY FL goat anti—mouse IgG antibody is labeling microtubules, and DAPI is labeling the nucleus.

Cell Analysis, Cellular Imaging, Immunofluorescence (IF)

Order Info

Shipping Condition: Room temperature

Specifications

Type Prepared Microscope Slide
Product Line BODIPY FluoCells Texas Red
For Use With (Application) Immunofluorescence
Content And Storage Store at room temperature and protect from light.
Quantity 1 Each
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?

Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.
DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

For Research Use Only. Not for use in diagnostic procedures.