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Molecular Probes™ EdU (5-ethynyl-2-deoxyuridine) DFS Item

Measures new DNA synthesis

$483.00 - $1,692.00

Specifications

Dye Type EdU
For Use With (Application) Measurement of DNA Synthesis
Storage Requirements Store in freezer (-5° to -30°C).
View More Specs

Disclaimers:

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.



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Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
A10044 Life Technologies
LSA10044
50mg Each for $483.00
E10187 Life Technologies
LSE10187
500mg Each for $1,692.00
Specifications

Description

EdU (5-ethynyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2'-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed with relevant antibody-based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS). Cell-Permeant.

Cell Analysis, Cell Cycle, Cell Proliferation, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Cell Cycle Assays, Flow Cytometry Cell Proliferation, Flow Cytometry of Cellular Processes, High-Content Screening (HCS)

Order Info

Shipping Condition: Room temperature

Description & Specifications

Specifications

EdU
Measurement of DNA Synthesis
Store in freezer (-5° to -30°C).