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Invitrogen™ Dihydrorhodamine 123

Catalog No. D23806
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D23806 1 mL
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Catalog No. D23806 Supplier Invitrogen™ Supplier No. D23806
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Description

Uncharged and nonfluorescent reactive oxygen species indicator

Dihydrorhodamine 123 can passively diffuse across membranes where it is oxidized to cationic rhodamine 123 which localizes in the mitochondria and exhibits green fluorescence.

Concentration: 5mM

Applications:

Apoptosis, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Free Radical Detection, Nitro-Oxidative Stress, Reactive Oxygen Species

Specifications

Quantity 1 mL

Frequently Asked Questions (FAQs)

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).
What is the excitation and emission wavelength for rhodamine?

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

Dye Excitation Emission EC
Rhodamine B 568 583 88,000
Rhodamine 123 507 529 101,000
Rhodamine 110 499 521 92,000
Rhodamine 6G 528 551 105,000
XRITC 572 596 92,000

For Research Use Only. Not for use in diagnostic procedures.