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Invitrogen™ Dihydroethidium (Hydroethidine)

Catalog No. D23107
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D23107 1 mL
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Catalog No. D23107 Supplier Invitrogen™ Supplier No. D23107

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Free radical sensor

The superoxide indicator dihydroethdium, also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.

Specifications

Quantity 1 mL
For Use With (Application) Superoxide Indicator
No. of Tests 1 mL
Detection Method Fluorescence
Excitation/Emission 518/606 nm
Product Type ROS Indicator
Excitation Wavelength Range 518 nm
Emission Wavelength Range 606 nm

Frequently Asked Questions (FAQs)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.
I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

For Research Use Only. Not for use in diagnostic procedures.