Invitrogen™ Di(Acetoxymethyl Ester) (6-Carboxy-2',7'-Dichlorodihydrofluorescein Diacetate)

6-carboxy-2',7'-dichlorodihydrofluorescein diacetate is nonfluorescent molecule is readily converted to green-fluorescent form when acetate groups are removed by intracellular esterases and oxidation (by the activity of ROS) occurs within cell. Upon cleavage of the acetate and ester groups by intracellular esterases and oxidation, both analogs form carboxydichlorofluorescein, with additional negative charges that impede its leakage out of cell.

• Ex/Em: approx. 492 - 495/517–527 nm
• Product is air sensitive (store under dry argon or nitrogen)
• Product may be dissolved in DMSO, DMF, or ethanol for use
• Indicator is cell permeant
• Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein
• Carboxylated H2DCFDA analog, which has two negative charges at physiological pH, and its di(acetoxymethyl ester) available separately

Refer to the Molecular Probes™ Handbook for additional product information.

Apoptosis, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Free Radical Detection, Nitro-Oxidative Stress, Reactive Oxygen Species