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Invitrogen™ Dextran, Rhodamine B, 10,000 MW, Neutral

Catalog No. D1824
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D1824 25 mg
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Catalog No. D1824 Supplier Invitrogen™ Supplier No. D1824
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Description

Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

  • Succinimidyl coupling of dyes to dextran molecule, which, in most cases, results in a neutral or anionic dextran
  • Suitable for neuronal tracing (anterograde and retrograde) in live cells
  • Cell lineage tracing in live cells
  • Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
  • Investigating vascular permeability and blood-brain barrier integrity
  • Monitoring acidification (some dextran-dye conjugates are pH-sensitive)
  • Studying hydrodynamic properties of the cytoplasmic matrix

  • Most of dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red™ dyes are essentially neutral

Cell Analysis, Cell Tracing and Tracking, General Cell Tracing, Neuronal Tracing

Order Info

Shipping Condition: Room temperature

Specifications

Product Type Dextran
Quantity 25 mg
Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Label or Dye Classic Dyes
Shipping Condition Room Temperature
Excitation/Emission 570/590 nm
Product Line Invitrogen

Frequently Asked Questions (FAQs)

What is the excitation and emission wavelength for rhodamine?

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

Dye Excitation Emission EC
Rhodamine B 568 583 88,000
Rhodamine 123 507 529 101,000
Rhodamine 110 499 521 92,000
Rhodamine 6G 528 551 105,000
XRITC 572 596 92,000

For Research Use Only. Not for use in diagnostic procedures.