missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Molecular Probes™ DAF-FM Diacetate (4-Amino-5-Methylamino-2',7'-Difluorofluorescein Diacetate) DFS Item

For detection and quantification of low concentrations of nitric oxide (NO)

$403.00 - $686.00

Specifications

Storage Conditions Store in freezer (-5 to -30°C) and protect from light.
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.



Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
D23842 Life Technologies
LSD23842
1mg Each for $686.00
D23844 Life Technologies
LSD23844
10 x 50μg Each for $403.00
Specifications

Description

It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.

  • Ex/Em of DAF-FM: approx. 495/515nm
  • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution
  • DAF-FM diacetate is cell permeant and passively diffuses across cellular membranes; once inside the cell, it is converted to cell-impermeant for
  • Buffers containing bovine serum albumin (BSA) and phenol red may affect fluorescence and should be used with caution
  • Fluorescence quantum yield of DAF-FM is approx. 0.005, but increases about 160-fold, to ~0.81, after reacting with NO
  • Assessment of NO production in transaldolase-deficient lymphoblasts by flow cytometry
  • Detection of NO accumulation in embryonic cortical neurons following neurotrophin stimulation
  • in vivo imaging of NO in zebrafish
I

  • ntravital microscopic detection of NO generation associated with angiogenesis in mice
  • Quantitation of ATP-induced NO release in rabbit platelets
  • Spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5
  • NO adduct of DAF-FM is significantly more photostable than that of DAF-2, which means additional time for image capture
  • DAF-FM is a more sensitive reagent for NO than is DAF-2 (NO detection limit for DAF-FM approx. 3nM versus approx. 5nM for DAF-2)

Refer to the Molecular Probes™ Handbook for additional product information.

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitric Oxide Research, Nitro-Oxidative Stress

Order Info

Shipping Condition: Room Temperature

Description & Specifications

Specifications

Store in freezer (-5 to -30°C) and protect from light.