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Molecular Probes™ DAF-FM Diacetate (4-Amino-5-Methylamino-2',7'-Difluorofluorescein Diacetate) DFS Item

For detection and quantification of low concentrations of nitric oxide (NO)

$385.35 - $665.70

Specifications

Storage Conditions Store in freezer (-5 to -30°C) and protect from light.
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

D23842

DFS Item
 
life technologies
D23842
1mg Each for $665.70

D23844

DFS Item
 
life technologies
D23844
10 x 50μg Each for $385.35
Description & Specifications

Specifications

Storage Conditions Store in freezer (-5 to -30°C) and protect from light.

It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.

  • Ex/Em of DAF-FM: approx. 495/515nm
  • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution
  • DAF-FM diacetate is cell permeant and passively diffuses across cellular membranes; once inside the cell, it is converted to cell-impermeant for
  • Buffers containing bovine serum albumin (BSA) and phenol red may affect fluorescence and should be used with caution
  • Fluorescence quantum yield of DAF-FM is approx. 0.005, but increases about 160-fold, to ~0.81, after reacting with NO
  • Assessment of NO production in transaldolase-deficient lymphoblasts by flow cytometry
  • Detection of NO accumulation in embryonic cortical neurons following neurotrophin stimulation
  • in vivo imaging of NO in zebrafish
I

  • ntravital microscopic detection of NO generation associated with angiogenesis in mice
  • Quantitation of ATP-induced NO release in rabbit platelets
  • Spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5
  • NO adduct of DAF-FM is significantly more photostable than that of DAF-2, which means additional time for image capture
  • DAF-FM is a more sensitive reagent for NO than is DAF-2 (NO detection limit for DAF-FM approx. 3nM versus approx. 5nM for DAF-2)

Refer to the Molecular Probes™ Handbook for additional product information.

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitric Oxide Research, Nitro-Oxidative Stress

Shipping Condition: Room Temperature