missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. ROS-Nitric Oxide Pathway Reagents and Kits
  6. D23841

Invitrogen™ DAF-FM (4-Amino-5-Methylamino-2',7'-Difluorofluorescein)

Catalog No. D23841
Encompass
Change view
Click to view available options
Quantity:
1 mg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
D23841 1 mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. D23841 Supplier Invitrogen™ Supplier No. D23841

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Use to detect and quantify low concentrations of nitric oxide

DAF-FM is a reagent that is used to detect and quantify low concentrations of nitric oxide (NO). It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.

DAF-FM Specifications:

  • Ex/Em of DAF-FM: ∼495/515 nm
  • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution
  • DAF-FM can be introduced into cells by pressure injection or perfusion from a patch-clamp pipette (choose DAF-FM diacetate (Cat. No. D23844) if you need a cell-permeant version)
  • Buffers containing bovine serum albumin (BSA) and phenol red may affect the fluorescence and should be used with caution
  • The fluorescence quantum yield of DAF-FM is ∼0.005, but increases about 160-fold, to ∼0.81, after reacting with NO

Applications of DAF-FM:

  • Assessment of NO production in transaldolase-deficient lymphoblasts by flow cytometry
  • Detection of NO accumulation in embryonic cortical neurons following neurotrophin stimulation
  • in vivo imaging of NO in zebrafish
  • Intravital microscopic detection of NO generation associated with angiogenesis in mice
  • Quantitation of ATP-induced NO release in rabbit platelets

Important Advantages of DAF-FM Over DAF-2

The spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5. Also, the NO adduct of DAF-FM is significantly more photostable than that of DAF-2, which means additional time for image capture. Finally, DAF-FM is a more sensitive reagent for NO than is DAF-2 (NO detection limit for DAF-FM ∼3 nM versus ∼5 nM for DAF-2).

Probes for Nitric Oxide Research

We offer several products for the detection of reactive oxygen species (ROS) and nitric oxide (NO). Review these products in Probes for Nitric Oxide Research—Section 18.3 in the Molecular Probes™ Handbook.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Order Info

Shipping Condition: Room Temperature

Specifications

Quantity 1 mg
For Use With (Application) NO Detection
Detection Method Fluorescence
Product Type NO Indicator
Excitation Wavelength Range 495 nm
Emission Wavelength Range 515 nm

For Research Use Only. Not for use in diagnostic procedures.