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Invitrogen™ EU (5-Ethynyl Uridine) DFS Item

Aids in detection of global RNA transcription

Manufacturer:  Invitrogen™ LSE10345

Catalog No. E10345



Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine. Approach uses powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or 'click' reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapten using the Click-iT Cell Reaction Buffer Kit. Cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS).

Cell Analysis, Click Chemistries, Labeling Chemistry, Metabolic Labeling and Nascent Protein Synthesis with Click Chemistry

Order Info

Shipping Condition: Room temperature



Store at ≤-20°C
Room Temperature
RNA Labeling
Click-iT™, Molecular Probes®
Other Label(s) or Dye(s)

For Research Use Only. Not for use in diagnostic procedures.