Invitrogen™ CellROX™ Orange Reagent, for oxidative stress detection 

Description

• Cell-permeant dye is non-fluorescent while in reduced state and exhibits bright orange fluorescence upon oxidation by reactive oxygen species (ROS), with absorption/emission maxima of ∽545/565 nm. While this reagent is not formaldehyde-fixable, it can be multiplexed with other live cell-compatible probes such as GFP, CellEvent™ Caspase 3/7 Green reagent, Image-iT™ DEAD Green, Green and Deep Red MitoTracker™ dyes, and NucBlue™ reagent. CellROXOrange Reagent is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), and flow cytometery as well as the Tali™ Image-based Cytometer.
• Compatible with other live cell probes, including GFP and may be added directly to serum-containing growth media
• Provided as stable, ready-to-use DMSO solution with simple protocol compatible with standard workflows in fluorescence microscopy
• Oxidative stress results from an imbalance between production ROS and ability of cells to scavenge them
• ROS play important role in progression of several diseases, including inflammation, atherosclerosis, aging, and age-related degenerative disorders
• Form: Frozen
• Excitation Class: Visible

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, High-Content Screening (HCS), Imaging Cytometry, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Nitro-Oxidative Stress, Reactive Oxygen Species

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