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Invitrogen™ Molecular Probes™ CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent DFS Item

Fluorogenic, no-wash indicator for live- and fixed-cell applications

Manufacturer:  Invitrogen™ LSR37111

Catalog No. R37111



Includes: 1 x 2.5mL dropper bottles

Description

Description

Activation of caspase-3 is an early indicator of apoptosis.

  • Allows rapid and sensitive detection of cells destined for cell death
  • Formulated as room temperature-stable solution and comes in convenient dropper bottle: tip and drip2 drops/mL to stain cells
  • Optimized caspase-3/7 substrate for apoptosis analysis
  • Simple and fast no-wash protocol helps preserve delicate apoptotic cells
  • Compatible with both live-cell fluorescence imaging and formaldehyde-based fixation methods
  • Ready-to-use liquid formulation: no need to dilute, weigh, or pipet
  • Four amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that is nonfluorescent when not bound to DNA: The Caspase-3/7 Green reagent is intrinsically nonfluorescent, as DEVD peptide inhibits binding of dye to DNA
  • Upon activation of caspase-3/7 in apoptotic cells, DEVD peptide is cleaved and free dye can bind DNA, generating bright green fluorescence
  • Apoptotic cells with activated caspase-3/7 will have bright green nuclei, while cells without activated caspase 3/7 will show minimal fluorescence
  • Fluorescent signal survives fixation and permeabilization, allowing end-point assays and immunocytochemistry-based methods
  • Optimization may be needed for some cell types, conditions, and applications: In such cases, simply add more or fewer drops until optimal staining intensity is obtained
  • If desired, combine with red or far-red nuclear stain for total cell count
  • Excited with maximum at 502nm and has emission maximum at 530nm
  • It is detected through standard GFP and FITC filters

Apoptosis, Apoptosis Related Factors, Caspase Activation, Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, High-Content Screening (HCS)

Specifications

Specifications

Confocal Microscope, Floid™ Cell Imaging System, Flow Cytometer, Fluorescence Microscope
Dropper Bottle(s)
Store at ≤ 25°C.
Green
Documents

For Research Use Only. Not for use in diagnostic procedures.