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Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit 
Obtain 10–50 times yield of conventional in vitro transcription reactions.
Supplier: Invitrogen™ AM1348
Description
mMESSAGE mMACHINE high yield capped RNA transcription kits use patented high-yield transcription technology for the routine synthesis of 15–35 μg of 7-methyl guanosine capped RNA from 1 μg of template DNA. Each kit includes sufficient reagents for 25 reactions.
- High yields of capped RNA—up to 35 μg of RNA per reaction
- Extra convenience—cap analog-nucleotide mix provided
- Simplified reaction format
- Synthesized transcripts ideal for microinjection, in vitro translation, or transfection
- 10–50 times yield obtained with conventional in vitro transcription reactions
- Ratio of cap analog to GTP has been optimized to allow compromise between yield (15–35 μg) and proportion of transcripts that are capped (80%)
- Contains a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
- Optimized for use with polymerases included in kit
- Contains 57 U of cap analog
Specifications
| T3 Enzyme Mix, 10X Reaction Buffer 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature. | |
| Kit | |
| In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products | |
| Ambion™, mMESSAGE mMACHINE™ | |
| 25 Reactions | |
| Dry Ice | |
| PCR Products, Linearized DNA plasmid |
| Synthetic mRNA, mRNA | |
| mRNA, Synthetic mRNA | |
| 25 Reactions | |
| T3 In Vitro Transcription kit | |
| T3 | |
| 20-30 μg |
For Research Use Only. Not for use in diagnostic procedures.