Thermo Scientific™ Middlebrook 7H10 Agar

Description

Cultivate and isolate Mycobacterium species with Thermo Scientific™ Remel™ Middlebrook 7H10 Agar. Based on the formulations developed initially by Dubos and Middlebrook¹, and modified by Middlebrook and Cohn², the Middlebrook 7H10 agar promotes the faster growth of Mycobacterium species and protects tubercle bacilli against a variety of toxic agents¹.

Middlebrook 7H10 Agar is a solid medium recommended for use in qualitative procedures for the cultivation and isolation of mycobacteria. Mycobacteria are immobile, slow-growing, rod-shaped, gram-positive bacteria with high genomic content. The genus includes pathogens known to cause severe diseases in mammals, including tuberculosis and leprosy.

• Promotes: the faster growth of Mycobacterium
• Selective: Malachite-green dye is a selective agent, which partially inhibits bacteria other than mycobacteria.
• Ready-to-use: the convenience of prepared media.

This medium contains inorganic salts which are essential to the growth of mycobacteria. Glycerol is a source of carbon and energy. Sodium citrate is converted to citric acid, which holds inorganic cations in solution. The malachite-green dye is a selective agent, which partially inhibits bacteria other than mycobacteria. Oleic Albumin Dextrose Catalase (OADC) Enrichment is a supplement which contains the following additives: sodium chloride to maintain osmotic equilibrium; dextrose, an energy source; catalase to destroy toxic peroxidases that may be present in the medium; oleic acid, which is required in the metabolism of mycobacterium; albumin to protect the tubercle bacilli against toxic agents.

Not all products are available for sale in all territories. Please inquire.

Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.