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Molecular Probes™ Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry

Catalog No. 501137622
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Catalog No. 501137622 Supplier Molecular Probes™ Supplier No. V35123
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Description

Vybrant™ Apoptosis Assay Kit #13 *PO-PRO™-1/7-aminoactinomycin D* *for flow cytometry* *200 assays*

This flow cytometry product detects apoptosis on the basis of changes that occur in the permeability of cell membranes using PO-PRO™-1 and 7-aminoactinomycin D (7-AAD) nucleic acid stains. After staining a cell population with PO-PRO™-1 dye and 7-AAD, apoptotic cells show violet fluorescence, dead cells show violet and red fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished by a flow cytometer that uses both a violet laser and the 488 nm line of an argon-ion laser for excitation. Each kit contains sufficient reagents for approximately 200 flow cytometry tests.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

Quantity 1 kit
Format Tube, Slide
Product Type Dead Cell Apoptosis Kit
Content And Storage Contains 1 vial of PO-PRO™-1 dye (500 μL), and 1 vial of 7-AAD (7-aminoactinomycin D, 200 μL).

Store in refrigerator (2–8°C) and protect from light.
Flow Cytometer Laser Lines 405, 488
Excitation/Emission PO-PRO™-1: 434⁄456, 7-AAD: 546⁄647
No. of Reactions 200
Shipping Condition Room Temperature
Product Line Vybrant
For Use With (Application) Flow Cytometry
Conjugate 7-AAD, PO-PRO-1
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
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For Research Use Only. Not for use in diagnostic procedures.