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Gibco™ MEM, no glutamine
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Catalog No. 11090099
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Catalog No. 11090099 Supplier Gibco™ Supplier No. 11090099
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Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells.

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle's salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type Primary Rat Astrocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type MEM (Minimum Essential Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives Low Glucose, Phenol Red
Without Additives No Glutamine, No HEPES, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 10 x 500 mL
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.