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  5. Invitrogen™ MEGAscript™ SP6 Transcription Kit

Invitrogen™ MEGAscript™ SP6 Transcription Kit

Catalog No. AM1330
Encompass
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Product Type:
SP6 In Vitro Transcription kit
Quantity:
40 Reactions
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Catalog No. Product Type Quantity
AM1330 SP6 In Vitro Transcription kit 40 Reactions
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Catalog No. AM1330 Supplier Invitrogen™ Supplier No. AM1330
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Description

Synthesize 10 to 50 times the amount of RNA produced by conventional transcription reactions

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions. Each Ambion kit includes sufficient reagents for 40 reactions.

  • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
  • Amplifies aRNA for gene array analysis and other applications
  • Efficiently incorporates many modified nucleotides
  • A typical 20μL MEGAscript reaction with 1μg of the pTRI-Xef-1α control template will yield over 100μg of transcript
  • Versatility of the MEGAscript kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase
  • Accessory Products:
  • MessageAmp™ aRNA Amplification Kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr. No. AM1908) and the MEGAclear-96 Kit (Mfr. No. AM1909).

Specifications

Content And Storage SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Final Product Type Synthetic mRNA, mRNA
Format Kit
Sample Type mRNA, Synthetic mRNA
For Use With (Application) In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions 40 Reactions
Product Line Ambion, MEGAscript
Product Type SP6 In Vitro Transcription kit
Quantity 40 Reactions
Promoter SP6
Shipping Condition Dry Ice
Yield 100 μg
Starting Material PCR Products, Linearized DNA plasmid
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Frequently Asked Questions (FAQs)

I am using the MEGAscript SP6 Transcription Kit for in vitro transcription. I ran my reaction product on a denaturing gel and got a smear. Can you offer some advice?

If the RNA appears degraded (e.g., smeared), remove residual RNase from the DNA template preparation before in vitro transcription. Do this by digesting the DNA prep with proteinase K (100-200 µg/mL) in the presence of 0.5% SDS for 30 min at 50°C, and follow this with phenol/chloroform extraction. The RNase Inhibitor that is present in the transcription reaction can only inactivate trace RNase contamination. Large amounts of RNase contamination will compromise the size and amount of transcription products.

For Research Use Only. Not for use in diagnostic procedures.