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MilliporeSigma™ MDR1 Efflux Assay
Includes two of the best characterized & most commonly used multidrug resistance ABC transporter substrates, DiOC2(3) and rhodamine 123
Supplier: MilliporeSigma™ ECM910
Includes:
- Sterile 5x RPMI-1640 - One bottle containing 200ml of sterile 5x concentrated RPMI-1640
- Sterile 30% BSA - One bottle containing 35ml sterile-filtered 30% bovine serum albumin (BSA) in PBS
- Gentamicin Solution, 1000x - One vial containing 1ml sterile-filtered 50mg/ml gentamicin
- DiOC2(3) Solution - One vial containing 50μl of DiOC2(3) (3,3'-diethyloxacarbocyanine iodide) at 1 mg/ml in DMSO
- Rhodamine 123 Solution - One vial containing 0.5ml of rhodamine 123 at 1mg/ml in DMSO
- Vinblastine Solution - One vial containing 150μl of 22mM vinblastine in DMSO
- DMSO - One vial containing 150μl DMSO
- Propidium Iodide Stock Solution - One vial containing 1ml of 50μg/ml propidium iodide in PBS
Description
The kit enables researchers to directly assess the functional activity of the MDR1, MRP1 and BCRP membrane pumps in living cells under physiologic conditions by directly measuring the relative fluorescence of cell populations that actively extrude fluorescent multidrug resistance transport substratesSpecifications
| Stored at 2°C to 8°C upon arrival | |
| DiOC2(3) and rhodamine 123 | |
| Stable for 1 year after receipt |
| Fluorescent | |
| 100 Assays |