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Gibco™ McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement
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Catalog No. 36600021
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36600021 500 mL
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Catalog No. 36600021 Supplier Gibco™ Supplier No. 36600021
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Description

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

With: High Glucose, GlutaMAX™, Bacto-peptone, Phenol Red

Without: Sodium Pyruvate

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.

Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Cell Type Mammalian Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type McCoy's 5A Medium
Sterility Sterile-filtered
With Additives High Glucose, GlutaMAX, Phenol Red, Bacto-peptone, Sodium Bicarbonate
Without Additives No HEPES, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco, GlutaMAX
Quantity 500 mL
Shipping Condition Room Temperature
Osmolality 280 - 320 mOsm/kg
pH 6.9 to 7.1
Content And Storage Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Culture Environment CO2
Culture Type Mammalian Cell Culture
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