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Invitrogen™ eBioscience™ MagniSort™ Human CD4 T cell 2-Step Enrichment Kit

Designed for magnetic separation of CD4+ T cells by positive selection after monocyte depletion

Supplier:  Invitrogen™ 8802683174

Catalog No. 501125064


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Alert:

Contains Sodium azide


Includes:
  • MagniSort Anti-Human CD14 Biotin : 200 tests, 20μL/test
  • MagniSort Anti-Human CD4 Biotin : 200 tests, 20μL/test
  • MagniSort Positive Selection Beads A

Description

Description

    Method
  • Monocytes are bound by Anti-Human CD14 antibody and then magnetic beads
  • When placed in a magnetic field, the monocytes are held in the magnet while the remaining cells are decanted and used for the second step
  • In the second step, the monocyte-depleted cells are bound by Anti-Human CD4 antibody and then magnetic beads
  • Now, when placed in the magnetic field, the CD4+ T cells are held in the magnet while undesired cells can be decanted
  • After enrichment, the purity of selected cells can be verified by staining with Anti-Human CD4, clones OKT4 or SK3
Specifications

Specifications

2° to 8°C. Do not freeze.
Magnetic Cell Separation
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
200 Tests
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