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Invitrogen™ eBioscience™ MagniSort™ Human CD4 T cell 2-Step Enrichment Kit

Catalog No. 501125064
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200 Tests
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Catalog No. Quantity
501125064 200 Tests
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Contains Sodium azide
Catalog No. 501125064 Supplier Invitrogen™ Supplier No. 8802683174
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Contains Sodium azide

Description

Includes

  • MagniSort Anti-Human CD14 Biotin : 200 tests, 20μL/test
  • MagniSort Anti-Human CD4 Biotin : 200 tests, 20μL/test
  • MagniSort Positive Selection Beads A

Designed for magnetic separation of CD4+ T cells by positive selection after monocyte depletion

    Method
  • Monocytes are bound by Anti-Human CD14 antibody and then magnetic beads
  • When placed in a magnetic field, the monocytes are held in the magnet while the remaining cells are decanted and used for the second step
  • In the second step, the monocyte-depleted cells are bound by Anti-Human CD4 antibody and then magnetic beads
  • Now, when placed in the magnetic field, the CD4+ T cells are held in the magnet while undesired cells can be decanted
  • After enrichment, the purity of selected cells can be verified by staining with Anti-Human CD4, clones OKT4 or SK3

Specifications

Content And Storage 2° to 8°C. Do not freeze.
For Use With (Application) Magnetic Cell Separation
Format Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Quantity 200 Tests