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Thermo Scientific™ MagnaBind™ Streptavidin Beads
Description
Thermo Scientific MagnaBind Streptavidin Beads are convenient for affinity purification or separation methods involving of biotin-labeled molecules. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.
Features of MagnaBind Streptavidin Beads:
- Composition: Silanized iron oxide
- Magnetization: 25-35EMU/g
- Type of Magnetization: Superparamagnetic (no magnetic memory)
- Surface Area: >100m2/g
- Bead Size: 1-4μm diameter
- Settling Rate: 4% in 30 minutes
- Effective Density: 2.5g/mL
- Number of Beads: 1 x 108 beads/mg
- pH Stability: Aqueous solution, above pH 4.0
- Concentration: 5 mg/mL
Streptavidin is a 60kDa protein from Streptomycetes avidinii. The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding. The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidine HCl, pH 1.5. Consequently, it is often possible to elute binding partners in an interaction complex without also eluting the biotinylated component.
Applications:
- Cell sorting using either positive or negative selection
- Protein purification or immunoassays using either direct or indirect methods
Specifications
Specifications
| Concentration | Slurry: 5 mg/mL |
| Content And Storage | Upon receipt store product at 4°C. Do not freeze product. Product is shipped at ambient temperature. |
| Description | MagnaBind Streptavidin Beads |
| Color | Brown |
| For Use With (Application) | Affinity Purification |
| Product Type | Streptavidin Magnetic Beads |
| Ligand Type | Streptavidin |
| Product Line | MagnaBind |
| Quantity | 5 mL |
| Volume (Metric) | 5 mL |
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Frequently Asked Questions (FAQs)
Pierce Streptavidin Magnetic Beads are homogeneous, non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles encapsulated with a proprietary polymer surface (no exposed iron, bead size of approximately 1 (0.5-1.5) µm, concentration 10 mg/mL). Pierce Streptavidin Magnetic Beads can be used in manual or automated applications (using KingFisher 96 and KingFisher Flex instruments, for example).
MagnaBind Streptavidin Beads are also superparamagnetic particles, but are heterogeneous aggregates made of silanized iron oxide (bead size approximately 1-4 µm, concentration 5 mg/mL). MagnaBind Streptavidin Beads can only be used in manual applications.
No, centrifuging causes the formation of irreversible aggregates which greatly reduces binding capacity.
No, freezing or drying will cause the beads to aggregate and lose binding activity. Store the beads only at 4 degrees C.
Thermo Scientific Streptavidin Magnetic Beads average about 1 micron in daimeter ranging from about 0.5-1.5 microns while MagnaBind Streptavidin Beads are heterogenous averaging 1 - 4 microns.
No, neither the MagnaBind Streptavidin Beads nor the Thermo Scientific Streptavidin Magnetic Beads are sterilized. However, they are both supplied in solutions containing sodium azide to prevent bacterial growth.
For Research Use Only. Not for use in diagnostic procedures.