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MilliporeSigma™ Magna Nuclear RIP™ (Native) Nuclear RNA-Binding Protein Immunoprecipitation Kit

Catalog No. 1710522
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24 Assays
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Catalog No. Quantity
1710522 24 Assays
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Catalog No. 1710522 Supplier MilliporeSigma™ Supplier No. 1710522
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Description

Includes

  • Nuclei Isolation Buffer
  • RIP Lysis Buffer
  • Protein A/G Magnetic Beads
  • RIP Lysis Buffer
  • 0.5M EDTA
  • DNase I (RNase Free) 2U/μL
  • DNase I Reaction Buffer
  • Protease Inhibitor Cocktail III, Animal Free
  • RNAse Inhibitor

Requires

  • Antibody of interest for RNA-binding protein immunoprecipitation (RIP)
  • Negative Control Antibody
  • Precipitation Carrier (e.g. Pellet Paint™ Co-Precipitant or linear Acrylamide (5mg/mL) if samples are intended for Next Gen Sequence library preparation
  • Reagents for qRT-PCR Analysis and/or Reagents for RNA-Seq Library Construction (optional)

Specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs

  • Generates cross-linked chromatin to allow analysis of a variety of chromatin-associated RNAs
  • Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 100,000 cells (native)
  • Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios
  • Suitable for analysis by RT-qPCR or RIP-seq
  • Complete set of reagents and detailed protocol to enable first-time success

Specifications

Content And Storage Store components at the temperatures indicated on the labels
For Use With (Application) RNA Binding Protein Immunoprecipitation (RIP), Protein Interaction Assays
Quantity 24 Assays
Stability Kit components are stable for 6 months from date of shipment when stored as directed