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  6. Invitrogen™ MagicMark™ XP Western Protein Standard
  7. LC5603

Invitrogen™ MagicMark™ XP Western Protein Standard DFS Item

Specifically designed for easy and convenient protein molecular weight estimation directly on Western blots

Supplier:  Invitrogen™ LC5603

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Catalog No. LC5603

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Includes: 50 μL of ready-to-use standard mixture is provided in a plastic vial; Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue

Description

Description

  • MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site
  • The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot
  • The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent)
  • The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use
  • Store at -20°C

Applications

  • Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel)
  • Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using western blot detection method
Specifications

Specifications

50 μL of ready-to-use standard mixture is provided in a plastic vial. Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue.

Store at -20°C.
9
Yes
Unstained
220, 120, 100, 80, 60, 50, 40, 30, 20 kDa
50 μL
Western Blotting, SDS-PAGE
User defined detection system
Protein Ladder
20 to 220 kDa
Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
MagicMark™
Dry Ice
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Product Information

Manuals

Protocol: MagicMark XP Western Protein Standard Version: MAN0007827 Rev.B.0 18Mar2014 File size: 587.8 kB
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For Research Use Only. Not for use in diagnostic procedures.