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  6. Invitrogen™ MagicMark™ XP Western Protein Standard

Invitrogen™ MagicMark™ XP Western Protein Standard

Catalog No. LC5602
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250 μL
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LC5602 250 μL
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Description

Includes

250 μL of ready-to-use standard mixture is provided in a plastic vial; Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue

Specifically designed for easy and convenient protein molecular weight estimation directly on Western blots

  • MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site
  • The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot
  • The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent)
  • The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use
  • Store at -20°C

Applications

  • Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel)
  • Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using western blot detection method

Order Info

Shipping Condition: Dry Ice

Specifications

Content And Storage 250 μL of ready-to-use standard mixture is provided in a plastic vial. Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue.

Store at -20°C.
Detection Method User defined detection system
Number of Markers 9
Ready to Load Yes
Size Range 20 to 220 kDa
Gel Compatibility Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol) 220, 120, 100, 80, 60, 50, 40, 30, 20 kDa
Quantity 250 μL
Shipping Condition Dry Ice
Product Line MagicMark
Product Type Protein Ladder
Stain Type Unstained
System Type Western Blotting, SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.