The Luna Cell Ready Probe One-Step RT-qPCR Kit provides all the necessary components for direct, probe-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. The Luna Cell Ready Probe One-Step RT-qPCR Kit is composed of: 1) the Luna Cell Ready Lysis Module and 2) the Luna Universal Probe One-Step RT-qPCR Kit. Coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis. The lysis capacity spans 10 -100,000 cells in a 50 l lysis reaction. Up to 2 l of lysate (equivalent to RNA from 0.2 - 4,000 cells) can be transferred into 20 l downstream RT-qPCR reactions.
