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Loading Solutions

Catalog No. K1026
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K1026 1 Kit
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Catalog No. K1026 Supplier Thermo Fisher Scientific Supplier No. K1026
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Loading Solutions

This kit contains the solutions needed to conveniently load the FRET-based, beta-lactamase substrates, CCF2-AM and CCF4-AM, into cells.

Specifications

Content And Storage Store at room temperature.
Product Line GeneBLAzer
Quantity 1 Kit
Shipping Condition Room Temperature
Type Loading Solution
Do you offer an alternative to GeneBLAzer In Vivo Detection Kit (Cat. No. 12578134)?

The following are two alternatives to the GeneBLAzer In Vivo Detection Kit:
- LiveBLAzer FRET-B/G Loading Kit with CCF2-AM (Cat. No. K1032, K1025)
- LiveBLAzer FRET-B/G Loading Kit with CCF4-AM (Cat. No. K1095, K1096, K1030)

Concerning CCF2-AM and CCF4-AM, these molecules differ by two carbons in the bridge that links the coumarin moiety to the lactam ring. Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells. CCF4-AM has better solubility properties than CCF2-AM and is thus best suited for screening applications. In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM.

Note: We also offer the loading solutions as a separate kit, Loading Solutions (Cat. No. K1026), and CCF2-AM is also available as a standalone product (Cat. No. K1023).

For Research Use Only. Not for use in diagnostic procedures.