Bioptechs™ Live Cell Microscopy Delta T™ Culture Dish System 

Description

For micro-observation and applications requiring physical access to cells or tissue: microinjection, electrostimulation, wounding, micromanipulation, mechanical probing, recording, and stimulation

• Accurate temperature control, uniform heat distribution, and high-numeric-aperture compatibility
• Works in confocal applications; adapts to specimens from monolayered adherent cells to tissue preparations, with inverted, water-immersion, and upright microscopes
• System passes ∽0.9w electrical charge through thin indium tin-oxide coating on underside of glass substrate where cells are grown, transferring heat through glass to cells
• Thermal regulation via instantaneous analog control of a feedback loop
• No peripheral heating: cells are unaffected by surface evaporation, heat is not lost to the atmosphere
• After plating, cells are ready for incubation or time-lapse imaging
• Controller has real-time temperature display (ambient to 50°C, accuracy ±0.1°C); compensates for surface evaporation
• Alarmed protection circuit safeguards cells. Dimensions: 151/2L x 6W x 3 in. H (39.4 x 15.2 x 7.6cm). Operates on 100–240V 50/60Hz or 24V battery

Delta T Culture Dish options

• Disposable polystyrene/glass dishes are available plain (when temperature control is not needed) or with electrically conductive, optically transparent coating and silver bus bars; 6mm depth optimizes access for microinjection and manipulation
• Contain self heating ITO coating, which provides first surface heat transfer directly to cells when using Delta T System
• Injection molded polystyrene sidewalls and lid, coverglass bottom
• Highest quality German Desag 263, low alkaline coverglass for optimum resolution
• O.D., 35mm, tapering to 23mm optical aperture
• Glass thickness of 0.17mm
• Available in clear and black; for fluorescence imaging, select black culture dishes
• Numerous accessories specific to these dishes allow for specialized applications including - media perfusion, gas perfusion, sample positioning, heating and cooling
• Optional stage adapters available to fit all major brands of microscopes, which custom stage adapters available
• Optional culturing cylinders may be used to barricade cells or suspended specimens in a dish; or to restrict and concentrate growth and location of cells plated on coverslip without use of grease