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Invitrogen™ Lens Culinaris (Edible Lentil) Agglutinin (LCA), fluorescein (FITC)

Catalog No. L32475
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5 mg
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L32475 5 mg
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Catalog No. L32475 Supplier Invitrogen™ Supplier No. L32475
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Description

Lens Culinaris Agglutinin (LCA), fluorescein ( FITC), is a bright-green LCA-fluorophore conjugate that is sourced from a common edible lentil.

LCA specificity is similar to concanavalin A, but narrower, resulting in differences in patterns of tissue staining. Wheat germ agglutinin, concanavalin A, and LCA are known to block inhibitory effects of nerve growth factor in cell-free systems.

• Very bright FITC conjugate detected with traditional FITC/GFP filter set, Ex/Em: 495/515 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: mannose, glucose
• Inhibiting/eluting sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
• Blood group specificity: non-specific
• Unconjugated lectin molecular weight: 50 KDa
• Immunofluorescence working concentration: 5–20 μg/mL

LCA-FITC is provided at a 5 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+.

If a precipitate forms upon long-term storage at 4°C, warm to 37°C and centrifuge before use.

Specifications

Conjugate FITC
Quantity 5 mg
Source Lentil
Content And Storage When stored at or below 2°C to 8°C in the dark, the product is stable for 6 months after receipt.
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Form Solution
Protein Family Lectins

For Research Use Only. Not for use in diagnostic procedures.