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Gibco™ L-Selenomethionine

Catalog No. A39247
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250 mg
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A39247 250 mg
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Catalog No. A39247 Supplier Gibco™ Supplier No. A39247
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Description

L-Selenomethionine is a naturally occurring amino acid containing selenium that is commonly used for the labeling of proteins to facilitate structural determination by x-ray crystallography.

Selenomethionine is an antioxidant and has been shown to increase glutathione peroxidase activity in endothelial cells

Specifications

Chemical Name or Material (S)-2-Amino-4-(methylseleno)butyric acid
Molecular Formula C5H11NO2Se
MDL Number MFCD00037210
InChI Key RJFAYQIBOAGBLC-BYPYZUCNSA-N
SMILES C[Se]CC[C@H](N)C(O)=O
IUPAC Name (2S)-2-amino-4-(methylselanyl)butanoic acid
Molecular Weight (g/mol) 196.12
Quantity 250 mg
Percent Purity >98%
Physical Form Powder
Shipping Condition Wet Ice
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Frequently Asked Questions (FAQs)

I am performing L-selenomethionine labeling and getting very low protein yields. What could be going wrong?

L-selenomethionine is a known cell toxin. Therefore, it is important to grow cells in the present of this amino acid for the shortest amount of time required to achieve maximal labeling. We recommend adding L-selenomethionine to the culture just prior to transfection (in Expi293 Met (-) Expression Medium). You may also starve cells for 6 hr by culturing cells in Expi293 Met (-) Expression Medium (without supplementation) and then proceed with L-selenomethionine add-back and transfection.

For Research Use Only. Not for use in diagnostic procedures.