Alexa Fluor™ Kinase Tracer 314, 25 μM

Numéro de catalogue. PV6087

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25 μL

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Numéro de catalogue.	Quantity
PV6087	25 μL

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Numéro de catalogue. PV6087 Fournisseur Alexa Fluor™ Code fournisseur PV6087

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Description

Alexa Fluor™ Kinase Tracer 315 is detected by addition of a Eu-labeled anti-tag antibody.

Excitation/Emission (nm): 340/615, 665
Label or Dye: Alexa Fluor™ 647
Target Enzyme: Kinases (Multiple)
Validated Kinases: PIK3CD, PIK3CG, FRAP1 (mTOR)
In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 314 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

Assay	Kinase Binding Assay
Assay Entry	Biochemical binding/kinetic
Content And Storage	Store at -20°C.
Excitation/Emission	340/615, 665
For Use With (Application)	TR-FRET
For Use With (Equipment)	Microplate Reader
Format	384-well Plate
Label or Dye	Alexa Fluor™ 647
Quantity	25 μL
Readout	Kinetic
Shipping Condition	Dry Ice
Target Entry	PIK3CG, PIK3CD/PIK3R1, mTOR, FRAP1
Type	Kinase Tracer
Validated Kinases	PIK3CD, PIK3CG, FRAP1 (mTOR)
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For Research Use Only. Not for use in diagnostic procedures.