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Alexa Fluor™ Kinase Tracer 1710, 25 μM
Alexa Fluor™ Kinase Tracer 1710 is detected by addition of a Eu-labeled anti-tag antibody.
Supplier: Alexa Fluor™ PV6088
Description
- Excitation/Emission (nm): 340/615, 665
- Label or Dye: Alexa Fluor™ 647
- Target Enzyme: Kinases (Multiple)
- Validated Kinases: PIK3CA, ABL1, ABL1 E255K, ABL1 G250E, ABL1 Y253F, WNK3
- In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 1710 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.
Specifications
| Kinase Binding Assay | |
| Store at -20°C. | |
| TR-FRET | |
| 384-well Plate | |
| 25 μL | |
| Dry Ice | |
| Kinase Tracer |
| Biochemical binding/kinetic | |
| 340/615, 665 | |
| Microplate Reader | |
| Alexa Fluor™ 647 | |
| Kinetic | |
| ABL1 G250E, ABL1 E255K, ABL1 Y253F, ABL1, PIK3CA/PIK3R1, WNK3 | |
| PIK3CA, ABL1, ABL1 E255K, ABL1 G250E, ABL1 Y253F, WNK3 |
For Research Use Only. Not for use in diagnostic procedures.