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Alexa Fluor™ Kinase Tracer 1710, 25 μM DFS Item

Alexa Fluor™ Kinase Tracer 1710 is detected by addition of a Eu-labeled anti-tag antibody.

Supplier:  Alexa Fluor™ PV6088

Numéro de catalogue. PV6088


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Description

Description

  • Excitation/Emission (nm): 340/615, 665
  • Label or Dye: Alexa Fluor™ 647
  • Target Enzyme: Kinases (Multiple)
  • Validated Kinases: PIK3CA, ABL1, ABL1 E255K, ABL1 G250E, ABL1 Y253F, WNK3
  • In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 1710 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.
Spécifications

Spécifications

Kinase Binding Assay
Store at -20°C.
TR-FRET
384-well Plate
25 μL
Dry Ice
Kinase Tracer
Biochemical binding/kinetic
340/615, 665
Microplate Reader
Alexa Fluor™ 647
Kinetic
ABL1 G250E, ABL1 E255K, ABL1 Y253F, ABL1, PIK3CA/PIK3R1, WNK3
PIK3CA, ABL1, ABL1 E255K, ABL1 G250E, ABL1 Y253F, WNK3
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For Research Use Only. Not for use in diagnostic procedures.