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Ion 550™ Kit—Chef
Description
The Ion 550 Kit contains pre-packaged template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of eight loaded chips with complete run traceability.
The Ion 550 Kit, together with the Ion 550 Single Chip Supplemental Kit, enables the flexibility to prepare one or two loaded chips per Ion Chef run, without loss of templating reagents.
The Ion 550 Chip workflow:
- Provides template preparation and chip loading reagents for up to 200 base–read libraries
- Maximizes flexibility with the ability to load one or two Ion 550 chips per Ion Chef run
- Simplifies your research with ready-to-use, disposable reagent cartridges
- Delivers fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
- Leverages the latest advancements in data quality and accuracy
The Ion GeneStudio S5 Plus and S5 Prime systems, along with the Ion Chef Instrument, provide reproducible sequencing workflows. Delivering up to 130 million reads in a single run, the Ion 550 Chip empowers rapid exome and transcriptome analysis, as well as high coverage for cell free nucleic acid applications. Combined with Ion AmpliSeq technology and Ion Reporter Software for data interpretation, the Ion 550 Chip workflow provides a cost-effective and comprehensive solution for your inherited disease or oncology research needs.
Specifications
Specifications
| Product Line | Ion Torrent |
| Content And Storage | • 1 Ion 550 Chef Reagents, store at -20°C • 1 Ion 550 Chef Solutions, store at room temperature • 4 Ion S5 Chef Supplies, store at room temperature • 1 Ion S5 Sequencing Solutions, store at room temperature • 1 Ion S5 Sequencing Reagents, store at -20°C |
| For Use With (Application) | Comparative Sequencing (Germline Mutations - 50:50) |
| For Use With (Equipment) | Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime, Ion S5 XL Sequencing System |
| Product Type | Chef Kit |
| Quantity | 8 reactions |
| Shipping Condition | Approved for shipment at Room Temperature or on Wet Ice |
| Throughput | 200 Base-Read Libraries |
Frequently Asked Questions (FAQs)
Yes, Ion S5 Sequencing Reagents (Part No. A27768) is used on the instrument for both Ion 540 and Ion 550 Chip sequencing.
In most cases, a chip type mismatch error indicates that the Ion Chef S5 Series Chip Balance was placed incorrectly in the Chip-loading centrifuge. Please make sure that the Ion Chef S5 Series Chip Balance is placed in Position 2 of the Chip-loading centrifuge.
To use the flexible workflow feature (single chip/dual chip) and enable cartridge-use tracking between Torrent Servers across multiple Ion Chef instruments, Torrent Servers must be linked in an Ion Mesh configuration. For further information on how to link Torrent Servers in an Ion Mesh setup, go to Software Administration -> Set up Ion Mesh in Torrent Suite Software Help.
The Ion 550 Kit-Chef is compatible with Torrent Suite Software version 5.8 and later.
The Ion 550 Kit-Chef can be used with up to 200-base-read libraries of any type, prepared using any available Ion library kit.