missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Research and Clinical Analyzers and Instruments
  4. DNA Sequencing Systems
  5. Ion 550™ Chip Kit

Ion 550™ Chip Kit
GREENER_CHOICE

Catalog No. A34538
Encompass
Change view
Click to view available options
Quantity:
4 Chips
8 Chips
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A34538 8 Chips
A34537 4 Chips
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. A34538 Supplier Thermo Fisher Scientific Supplier No. A34538

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

The Ion 550 Chip Kit contains four (Cat. No. A34537) or eight (Cat. No. A34538) barcoded chips for sample tracking and sequencing with the Ion GeneStudio S5 Plus, the Ion GeneStudio S5 Prime, and Ion S5 XL sequencing systems. The Ion 550 Chip generates 100–130 million sequencing reads by electronically detecting polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of as little as 2.5 hours for 200 bp sequencing.

The Ion 550 Chip Kit offers:
• Support for a variety of research applications, including sequencing of up to four human exomes, cell-free nucleic acid applications, transcriptome profiling, and targeted resequencing of gene panels
• A rapid run-time of as little as 2.5 hours for 200 bp sequencing
• Sample tracking functionality from an integrated barcode
Notes:The Ion 550 Chip is only compatible with the Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime, and Ion S5 XL systems.
Use this chip with the Ion 550 Kit—Chef for automated template preparation and chip loading.

Specifications

Product Line Ion Torrent
Content And Storage Store at room temperature.
For Use With (Application) Comparative Sequencing (Germline Mutations - 50:50)
For Use With (Equipment) Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime, Ion S5 XL Sequencing System
Product Type Chip Kit
Quantity 8 Chips
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Why did my Ion Chef Instrument stop during a run?

- The instrument may have encountered an error. We recommend recording the error shown on the instrument display, then tapping OK. For further assistance, contact Technical Support at techsupport@thermofisher.com.
- A consumable may have been improperly loaded. We recommend ensuring that all consumables are correctly loaded. For instructions, see the user guide for your templating kit. For further assistance, contact Technical Support at techsupport@thermofisher.com.
Why is the door of the Ion Chef Instrument not opening?

- An obstruction may be present on or around the door mechanism. We recommend removing the obstruction blocking the door, then resuming normal instrument operation.
- The instument may be experiencing a hardware or software error. We recommend contacting Technical Support at techsupport@thermofisher.com.C8
Why does the Ion Chef Instrument not display the home screen when powered on?

Multiple causes are possible. We recommend powering off the instrument, waiting for 30 secs and then powering on the instrument. If you continue to experience the issue, contact Technical Support at techsupport@thermofisher.com.
How long can my loaded Ion 550 chip be stored prior to running on the sequencer?

Remove and sequence chips as soon as possible after the Ion Chef System finishes loading them. If you cannot sequence a loaded chip immediately, store it in a chip storage container at 4 degrees C until you are ready to run it (up to 24 hours maximum).
Why do my Ion 550 chip "Reagent Check" traces look different from those seen with the other Ion chips (510, 520, 530, and 540)?

This is because the new chip surface and the way the instrument reads the Ion 550 chip are different from the Ion 540/530/520/510 chips.
How many Oncomine Comprehensive Assay v3 libraries may be pooled on a single Ion 550 chip?

The Oncomine Comprehensive Assay v3 is validated with 32 libraries per Ion 550 chip (16 samples DNA and RNA).
How many Ion AmpliSeq Human Exome libraries can be pooled on a single Ion 550 chip?

The Ion 550 workflow supports 3-4 Ion AmpliSeq Human Exome libraries per run.
What are the shipping and storage conditions for the Ion 550 chip?

The Ion 550 chip is shipped at room temperature and should be stored at room temperature.
Which instrument platforms can I use with the Ion 550 chip?

The Ion 550 Chip is compatible with the Ion GeneStudio S5 Plus System, Ion GeneStudio S5 Prime System, and Ion S5 XL System. The Ion GeneStudio S5 System and Ion S5 System do not have enough computing power to work with the Ion 550 chip.
Can I perform templating with either the Ion OneTouch 2 System or the Ion Chef System when using the Ion 550 chip for sequencing?

The Ion 550 chip workflow is only supported with the Ion Chef System for templating.
What is the largest length I can sequence with an Ion 550 chip?

Up to 200 bp libraries (500 flows) are supported on the Ion 550 chip.
How many reads can I expect with an Ion 550 chip?

You may expect 100-130 million reads with an Ion 550 chip.