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Invitrogen™ UltraPure™ Ethidium Bromide, 10 mg/mL

Catalog No. 15585011
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Catalog No. 15585011 Supplier Invitrogen™ Supplier No. 15585011
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Description

Includes

10mL of 10mg/mL solution

Sensitive fluorescent dye detects nucleic acids in agarose gels and cesium chloride gradients

UltraPure Ethidium Bromide is available as an aqueous solution containing 10mg/mL UltraPure ethidium bromide and high-purity water. Sensitive: Detects as little as little as 1ng of nucleic acids in agarose gels.

  • Versatile: Detects DNA or RNA
  • Easy to Use: Can be used undiluted or diluted to the desired concentration
  • An aromatic compound with a phenanthridine core, ethidium bromide has excitation maxima at 300nm and 520nm and an emission maximum at 600nm

Agarose Gel Electrophoresis, DNA & RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis & Blotting

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage 10ml of 10mg⁄ml solution. Store at 15°C to 30°C
Detection Location In-Gel Detection
Detection Method Fluorescence
Label or Dye Ethidium Bromide
Quantity 10 mL
Shipping Condition Room Temperature
Target Molecule RNA, DNA
Product Line UltraPure
Product Type Ethidium Bromide

Frequently Asked Questions (FAQs)

Do you have a protocol for ethidium bromide staining in agarose gels?

For use in agarose gels:

- Add ethidium bromide to melted agarose to a final concentration of 0.5 µg/mL. Do not melt agarose that already contains ethidium bromide.

For staining agarose gels after electrophoresis:

- You can stain gels that have been run in the absence of ethidium bromide by covering the gel in 0.5 µg/mL ethidium bromide in water and gently agitating for 10 to 30 minutes.
- If necessary, gels can be destained by shaking in H2O for an additional 30 minutes. To stain RNA gels, you should minimize staining time and definitely include a destaining period.

What can ethidium bromide be used to detect and what is its sensitivity?

Ethidium bromide can be used to detect ssDNA, RNA, and dsDNA. This fluorescent dye intercalates between the stacked bases of nucleic acids, and exhibits an increased fluorescence at 590 nm. Ethidium bromide can detect down to ~1-10 ng/band of dsDNA in an agarose gel.

What amount of ethidium bromide do I need to stain nucleic acids in gels?

After electrophoresis, the gel should be stained in a 0.5-1.0 µg/mL solution of ethidium bromide in deionized water for 15 to 60 min depending on the thickness of the gel. As an optional step to reduce background fluorescence, the gel can be destained in deionized water for 15 to 30 min. Alternatively, ethidium bromide may be added directly to the agarose prior to casting. Add ethidium bromide to melted agarose to a final concentration of 0.5 µg/mL. This has the advantage of reducing the amount of ethidium bromide waste. However, this procedure may reduce the migration rate of nucleic acids.

For Research Use Only. Not for use in diagnostic procedures.