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Invitrogen™ T7 RNA Polymerase DFS Item

DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoter sequences

$347.00 - $626.00

Specifications

Product Type Polymerase, T7 RNA
For Use With (Application) Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis
Includes T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200mM Tris-HCl (pH 8.0), 40mMmgCl2, 10mM spermidine-(HCl)3, 125mM NaCl], vial of 100mM DTT
Quantity 2500 units
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200mM Tris-HCl (pH 8.0), 40mM MgCl2 , 10mM spermidine-(HCl)3 , 125mM NaCl], vial of 100mM DTT.


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
18033019 Life Technologies
LS18033019
2500 units Each for $347.00
18033100 Life Technologies
LS18033100
2 x 2500 units Each for $626.00
Specifications

Description

Synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.

  • Application: Synthesis of labeled and unlabeled RNA transcripts.
  • Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid
  • Performance and Quality Testing: 3' and 5' exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction
  • Unit Definition: One unit hydrolyzes 1nMol of ribonucleotide into acid-precipitable material in 1 hr at 37°C using a T7 transcription vector as template
  • Unit Reaction Conditions: 40mM Tris-HCl (pH 8.0), 25mM NaCl, 8mM MgCl2, 2mM spermidine-(HCl)3, 5mM DTT, 0.4mM ATP, 0.4mM CTP, 0.4mM GTP, 0.4mM UTP, 1μCi [3H]GTP, 1μg SphI-cut pT7L13, and enzyme in 50μL for 10 min. at 37°C

Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Wet Ice

Description & Specifications

Specifications

Polymerase, T7 RNA
Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis
T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200mM Tris-HCl (pH 8.0), 40mMmgCl2, 10mM spermidine-(HCl)3, 125mM NaCl], vial of 100mM DTT
2500 units