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  6. Invitrogen™ SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO

Invitrogen™ SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO

Catalog No. S7586
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500 μL
1 mL
20 x 50 μL
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S7586 20 x 50 μL
S7564 500 μL
S7568 1 mL
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Catalog No. S7586 Supplier Invitrogen™ Supplier No. S7586
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Description

Displays bright fluorescence when bound to RNA and low background in gels. Invitrogen™ SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO is ideal for use with either formaldehyde/agarose or polyacrylamide gels.

SYBR Green II RNA gel stain is one of the most sensitive dyes known for detecting RNA in electrophoretic gels using laser scanners or standard UV transilluminators. It is significantly more sensitive than ethidium bromide, the most commonly used stain for detecting nucleic acids in gels.

Available in three convenient packaging options:

  • 500μL (stains 50 minigels)
  • 1mL (stains 100 minigels)
  • 20 × 50μL (each stains 5 minigels)—Avoids waste when processing fewer minigels

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage • Supplied as a 10,000X concentrate in DMSO

Store at -20°C, protected from light in a dessicator.
Detection Location In-Gel Detection
Detection Method Fluorescence
Label or Dye SYBR Green II
Quantity 20 x 50 μL
Shipping Condition Room Temperature
Target Molecule RNA
Product Type RNA Gel Stain

Frequently Asked Questions (FAQs)

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain was specifically developed as a safer alternative to ethidium bromide. SYBR Green I is an ultrasensitive stain for dsDNA, and SYBR Green II is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

What are the differences between all the SYBR dyes?

Different SYBR dyes bind to dsDNA, ssDNA, and RNA, but vary in the sensitivity and specificity with which they bind to different nucleic acids. SYBR Green I Nucleic Acid Gel Stain is used for staining dsDNA and ssDNA. SYBR Green II RNA Gel Stain will stain dsDNA and ssDNA but has better sensitivity for RNA. SYBR Gold Acid Gel Stain was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain offering the highest sensitivity for both DNA and RNA. SYBR Safe DNA Gel Stain is a reduced mutagenicity formula designed for use with blue light systems. It is less sensitive than the SYBR Green I and II but comparable to ethidium bromide.

Please check the following link for additional details regarding the different SYBR dyes: The Molecular Probes Handbook: Nucleic Acid Detection on Gels, Blots and Arrays
Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

For Research Use Only. Not for use in diagnostic procedures.