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Invitrogen™ SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)

Catalog No. S11494
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S11494 500 μL
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Catalog No. S11494 Supplier Invitrogen™ Supplier No. S11494
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Description

Includes

Supplied as a 10,000X concentrate in DMSO.

Extremely sensitive fluorescent stain for detection of nucleic acids

Use with UV transilluminators, the Dark Reader, laser scanners, or blue light transilluminators.

  • Ultra Sensitive: 25–100 times more sensitive than ethidium bromide. Detect as little as 25pg of DNA
  • Improved DNA Cloning Efficiency: Excitable with blue light transillumination, which does not cause DNA damage
  • Increased Signal to Background Ratio: More than 1000-fold signal enhancement when bound to nucleic acids
  • Versatile: Detects dsDNA, ssDNA and RNA in native, glyoxal, formaldehyde or urea gels

Stain Properties SYBR Gold stain is a proprietary unsymmetrical cyanine dye that exhibits >1000-fold fluorescence enhancement upon binding to nucleic acids and has a high quantum yield (∽0.6) upon binding to double- or single-stranded DNA or to RNA1. Excitation maxima for dye-nucleic acid complexes are at ∽495nm and ∽300nm and the emission maximum is ∽537 nm.

Stain Sensitivity SYBR Gold stain is >10-fold more sensitive than ethidium bromide for detecting DNA and RNA in denaturing urea, glyoxal, and formaldehyde gels, even with 300nm transillumination. SYBR Gold stain has also been shown to be much more sensitive than SYBR Green II stain for detecting single strand conformation polymorphism (SSCP) products and is much more sensitive than silver-staining for the detection of double-stranded DNA in native polyacrylamide gels.

Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting

Order Info

Shipping Condition: Room temperature

1Tuma RS, Beaudet MP, Jin X, Jones LJ, Cheung CY, Yue S, Singer VL. Characterization of SYBR Gold nucleic acid gel stain: a dye optimized for use with 300-nm ultraviolet transilluminators. Anal Biochem (1999) 268:278-288. (PMID: 10075818)

Specifications

Content And Storage • Supplied as a 10,000X concentrate in DMSO

Store at -20°C, protected from light in a dessicator.
Detection Location In-Gel Detection
Detection Method Fluorescence
Label or Dye SYBR Gold
Quantity 500 μL
Shipping Condition Room Temperature
Target Molecule RNA, DNA
Product Type Nucleic Acid Gel Stain

Frequently Asked Questions (FAQs)

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain was specifically developed as a safer alternative to ethidium bromide. SYBR Green I is an ultrasensitive stain for dsDNA, and SYBR Green II is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

How should I dispose of SYBR DNA Gel Stain?

Disposal regulations vary. Please contact your safety office or local municipality for disposal guidelines.

What are the differences between all the SYBR dyes?

Different SYBR dyes bind to dsDNA, ssDNA, and RNA, but vary in the sensitivity and specificity with which they bind to different nucleic acids. SYBR Green I Nucleic Acid Gel Stain is used for staining dsDNA and ssDNA. SYBR Green II RNA Gel Stain will stain dsDNA and ssDNA but has better sensitivity for RNA. SYBR Gold Acid Gel Stain was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain offering the highest sensitivity for both DNA and RNA. SYBR Safe DNA Gel Stain is a reduced mutagenicity formula designed for use with blue light systems. It is less sensitive than the SYBR Green I and II but comparable to ethidium bromide.

Please check the following link for additional details regarding the different SYBR dyes: The Molecular Probes Handbook: Nucleic Acid Detection on Gels, Blots and Arrays
What is the stability of SYBR Green I and SYBR Gold Nucleic Acid gel stains?

When stored frozen at -20 degrees C or lower, desiccated and protected from light, the SYBR Green I stain and SYBR Gold stain stock solutions in DMSO are stable for one year from date of shipment.
Working solutions prepared in the recommended manner can be stored in an airtight, light-protected (foil wrapped or dark) polypropylene container in the refrigerator between uses. Under these conditions, working solutions will reliably last 3 or 4 days.
Staining solutions prepared in buffers with pH below about 7.0 or above 8.5 are less stable and show reduced staining efficacy.
Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity.

We recommend storing aqueous working solutions in plastic rather than glass, as the stain may adsorb to glass surfaces.
Can I use SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) (Cat. No. S11494) to detect RNA in gels?

SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) (Cat. No. S11494) can detect dsDNA, ssDNA, and RNA in native, glyoxal, formaldehyde or urea gels.
Can I detect a 5-nucleotide long ssDNA with SYBR Gold Nucleic Acid Gel Stain (Cat. No. S11494)?

SYBR Gold can detect 12-18 mer ssDNA. It has not been tested on a smaller oligo.
Can I use the Dual LED Blue/White Light Transilluminator to visualize gels stained with SYBR Safe DNA Gel Stain or SYBR Gold Nucleic Acid Gel Stain?

Yes, the Dual LED Blue/White Light Transilluminator can be used to visualize gels stained with SYBR Safe DNA Gel Stain, SYBR Gold Nucleic Acid Gel Stain, or Ethidium bromide.

For Research Use Only. Not for use in diagnostic procedures.