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Invitrogen™ Dynabeads™ Untouched™ Human B Cells Kit

Numéro de catalogue. 11351D
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11351D 1 kit
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Numéro de catalogue. 11351D Fournisseur Invitrogen™ Code fournisseur 11351D
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Description

Comprend

10mL Depletion MyOne; SA Dynabeads and 2mL Antibody Mix with biotinylated monoclonal antibodies towards human CD2, CD14, CD16 (a and b), CD36, CD43 and CD235a (Glycophorin A)

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes.

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched B cells
• Easy to use and to scale up
• No columns required

An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC

Order Info

Shipping Condition: Room Temperature

Spécifications

Cell Type B Cells
Content And Storage The Kit Contains: 10 mL Depletion MyOne™ SA Dynabeads™ and 2 mL Antibody Mix with biotinylated monoclonal antibodies towards human CD2, CD14, CD16 (a and b), CD36, CD43 and CD235a (Glycophorin A).

Store at 2°C to 8°C.
Product Type Cell Isolation Kit
Final Product Type Cells
Format Bead
Isolation Technology Negative Isolation
No. of Cells Processes ∼1 x 109 cells/kit
Output Viability >97%
Product Line DYNAL, Dynabeads, Untouched
Purity or Quality Grade Research Grade
Quantity 1 kit
Sample Type MNC
Shipping Condition Room Temperature
Starting Material Cell No. ∼5 x 107 cells/test
Target Species Human
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For Research Use Only. Not for use in diagnostic procedures.